FITC標(biāo)記番茄凝集素
FITC labeled Lycopersicon Esculentum (Tomato) Lectin
產(chǎn)品編號 | 產(chǎn)品名稱 | 包裝規(guī)格 | 價格 |
NBS8021-500ug | FITC標(biāo)記番茄凝集素 | 500ug | 2125 |
產(chǎn)品簡介:
番茄凝集素(Tomato lectin,Lycopersicon Esculentum Lectin(LEL)),是一種非常穩(wěn)定的單亞基糖蛋白,約含有等量的蛋白質(zhì)和碳水化合物。據(jù)報道其糖類部分由85%阿拉伯糖和15%半乳糖組成,在溶液中可能形成多聚體聚合物。LEL無血型特異性,但能親和N-乙酰-β-D-葡糖胺寡聚體。LEL能夠很好的結(jié)合內(nèi)皮細(xì)胞,用來觀察和評價微血管系統(tǒng)。LEL能夠抑制菜豆來源植物血凝素(PMA)的促有絲分裂活性。
本品為FITC標(biāo)記的番茄凝集素(來源于Lycopersicon esculentum),Ex/Em=495/515nm,通過血管內(nèi)灌注法或直接染色組織切片能快速且有效的檢測和可視化嚙齒類動物的血管和小膠質(zhì)細(xì)胞。
產(chǎn)品特性:
1)英文同義名:Lycopersicon esculentum agglutinin(LEA),FITC Conjugate; Fluorescein labeled Lycopersicon Esculentum (Tomato) Lectin; FITC-LEL; FITC-LEA;
2)分子量:~71kDa
3)糖類特異性:[GlcNAc]1-3, N-Acetylglucosamine
4)F/P(摩爾):2.0-5.0
5)外觀:黃色至黃綠色液體(溶于10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1 mM Ca2+, 0.08% sodium azide and 5 mg/mL β-cyclodextrin)
6)濃度:2mg/ml
7)Ex/Em=495/515nm
8)應(yīng)用:糖生物;IF;
保存條件:
2-8℃避光保存,1年有效。
產(chǎn)品使用:(源自文獻(xiàn),僅作參考)
動物模型(Animal Model):Xenografts were established in 4- to 6-week-old female NCR nude mice by intrarenal injection of 106 SK-NEP-1 cultured human Wilms tumor cells and allowed to grow. After 5 weeks, large tumors were palpable in all mice, and a cohort was randomly selected to provide day-0 controls.
灌注方法(Lectin perfusion):Selected mice at each time point underwent intravascular injection of fluorescein- labeled Lycopersicon esculentum lectin (100 μg in 100 μl of saline) into the left ventricle. The vasculature was fixed by infusion of 1% paraformaldehyde (pH 7.4) in PBS and then washed by perfusion of PBS.
給藥途徑(Administration):Intravascular (i.v.) injection
注意事項(xiàng):
1. 本品長期保存中可能產(chǎn)生沉淀,37℃溫育溶解后再使用。
2. 本品含防腐劑疊氮鈉,操作過程中請注意防護(hù)。
3. 為了您的安全和健康,請穿實(shí)驗(yàn)服并戴一次性手套操作。
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